Alternative Crops for Ethanol Fuel Production: Agronomic, Processing, and Economic Considerations

This report is a result of the fuel alcohol research team\u27s broadened focus during 1983. A comprehensive literature review was carried out to explore alternative starch and sugar crop alternatives for ethanol fuel production. Although the literature search was quite inclusive with respect to geographic regions, special emphasis was given to the agronomic and economic potential of various fuel alcohol crops in the Northern Plains region of the U.S., of which South Dakota is a part, and in LDCs of Asia, Africa, and Latin America. Our intent was to thereby determine possible energy crops deserving of more fuel alcohol research attention in the Northern Plains and also provide a document of use to ourselves and others considering various crops for fuel alcohol production in LDCs. Development assistance agencies, and universities such as SDSU which work with them, must be able to assess the energy producing potential of agricultural economies, along with food and fiber producing potentials. One kind of energy production that may be technically and economically feasible in some LDCs is fuel alcohol production from starch and sugar crops. (In this report, the terms alcohol and ethanol are used interchangeably.

MsbA-dependent translocation of lipids across the inner membrane of Escherichia coli

MsbA is an essential ABC transporter in Escherichia coli required for exporting newly synthesized lipids from the inner to the outer membrane. It remains uncertain whether or not MsbA catalyzes trans-bilayer lipid movement (i.e. flip-flop) within the inner membrane. We now show that newly synthesized lipid A accumulates on the cytoplasmic side of the inner membrane after shifting an E. coli msbA missense mutant to the non-permissive temperature. This conclusion is based on the selective inhibition of periplasmic, but not cytoplasmic, covalent modifications of lipid A that occur in polymysin-resistant strains of E. coli. The accessibility of newly synthesized phosphatidylethanolamine to membrane impermeable reagents, like 2,4,6-trinitrobenzene sulfonic acid, is also reduced severalfold. Our data showed that MsbA facilitates the rapid translocation of some lipids from the cytoplasmic to the periplasmic side of the inner membrane in living cells

Discrete phase space based on finite fields

The original Wigner function provides a way of representing in phase space the quantum states of systems with continuous degrees of freedom. Wigner functions have also been developed for discrete quantum systems, one popular version being defined on a 2N x 2N discrete phase space for a system with N orthogonal states. Here we investigate an alternative class of discrete Wigner functions, in which the field of real numbers that labels the axes of continuous phase space is replaced by a finite field having N elements. There exists such a field if and only if N is a power of a prime; so our formulation can be applied directly only to systems for which the state-space dimension takes such a value. Though this condition may seem limiting, we note that any quantum computer based on qubits meets the condition and can thus be accommodated within our scheme. The geometry of our N x N phase space also leads naturally to a method of constructing a complete set of N+1 mutually unbiased bases for the state space.Comment: 60 pages; minor corrections and additional references in v2 and v3; improved historical introduction in v4; references to quantum error correction in v5; v6 corrects the value quoted for the number of similarity classes for N=

PHA Productivity and Yield of Ralstonia eutropha

The research described in this present study was part of a larger effort focused on developing a dual substrate, dual fermentation process to produce Polyhydroxyalkanoate (PHA). The focus of this study was developing and optimizing a strategy for feeding a mixture of SCFAs (simulated ARF) and maximizing PHA production in a cost-effective way. Three different feeding strategies were examined in this study. The substrate evaluated in this study for the growth phase of R. eutropha was condensed corn solubles, a low-value byproduct of the dry-mill, corn ethanol industry. The culture was grown to high cell densities in nitrogen-supplemented condensed corn solubles media in 5 L bioreactors. The overall growth rate of R. eutropha was 0.2 h−1. The 20 mL ARF feeding every 3 h from 48 to 109 h strategy gave the best results in terms of PHA production. PHA productivity (0.0697 g L−1 h−1), PHA concentration (8.37 g L−1), and PHA content (39.52%) were the highest when ARF was fed every 3 h for 61 h. This study proved that condensed corn solubles can be potentially used as a growth medium to boost PHA production by R. eutropha thus reducing the overall cost of biopolymer production

Thermal divergences on the event horizons of two-dimensional black holes

The expectation value of the stress-energy tensor \langleT_{\mu\nu}\rangle of a free conformally invariant scalar field is computed in a general static two-dimensional black hole spacetime when the field is in either a zero temperature vacuum state or a thermal state at a nonzero temperature. It is found that for every static two-dimensional black hole the stress-energy diverges strongly on the event horizon unless the field is in a state at the natural black hole temperature which is defined by the surface gravity of the event horizon. This implies that both extreme and nonextreme two-dimensional black holes can only be in equilibrium with radiation at the natural black hole temperature.Comment: 13 pages, REVTe

Kaluza-Klein Black Holes in String Theory

Exact solutions of heterotic string theory corresponding to four-dimensional magnetic black holes in $N=4$ supergravity are described. The solutions describe the black holes in the throat limit, and consist of a tensor product of an $SU(2)$ WZW orbifold with the linear dilaton vacuum, supersymmetrized to $(1,0)$ world sheet SUSY. One dimension of the $SU(2)$ model is interpreted as a compactified fifth dimension, leading to a four dimensional solution with a Kaluza-Klein gauge field having a magnetic monopole background; this corresponds to a solution in $N=4$ supergravity, since that theory is obtained by dimensional reduction of string theory.Comment: 13p. uses Harvma

Astrophysical Bounds on Global Strings

Global topological defects produce nonzero stress-energy throughout spacetime, and as a result can have observable gravitational influence on surrounding matter. Gravitational effects of global strings are used to place bounds on their cosmic abundance. The minimum separation between global strings is estimated by considering the defects' contribution to the cosmological energy density. More rigorous constraints on the abundance of global strings are constructed by examining the tidal forces such defects will have on observable astrophysical systems. The small number of observed tidally disrupted systems indicates there can be very few of these objects in the observable universe.Comment: 14 pages, REVTe

Loss of Atrx Affects Trophoblast Development and the Pattern of X-Inactivation in Extraembryonic Tissues

ATRX is an X-encoded member of the SNF2 family of ATPase/helicase proteins thought to regulate gene expression by modifying chromatin at target loci. Mutations in ATRX provided the first example of a human genetic disease associated with defects in such proteins. To better understand the role of ATRX in development and the associated abnormalities in the ATR-X (alpha thalassemia mental retardation, X-linked) syndrome, we conditionally inactivated the homolog in mice, Atrx, at the 8- to 16-cell stage of development. The protein, Atrx, was ubiquitously expressed, and male embryos null for Atrx implanted and gastrulated normally but did not survive beyond 9.5 days postcoitus due to a defect in formation of the extraembryonic trophoblast, one of the first terminally differentiated lineages in the developing embryo. Carrier female mice that inherit a maternal null allele should be affected, since the paternal X chromosome is normally inactivated in extraembryonic tissues. Surprisingly, however, some carrier females established a normal placenta and appeared to escape the usual pattern of imprinted X-inactivation in these tissues. Together these findings demonstrate an unexpected, specific, and essential role for Atrx in the development of the murine trophoblast and present an example of escape from imprinted X chromosome inactivation

Loss of ATRX in Chondrocytes Has Minimal Effects on Skeletal Development

BACKGROUND:Mutations in the human ATRX gene cause developmental defects, including skeletal deformities and dwarfism. ATRX encodes a chromatin remodeling protein, however the role of ATRX in skeletal development is currently unknown. METHODOLOGY/PRINCIPAL FINDINGS:We induced Atrx deletion in mouse cartilage using the Cre-loxP system, with Cre expression driven by the collagen II (Col2a1) promoter. Growth rate, body size and weight, and long bone length did not differ in Atrx(Col2cre) mice compared to control littermates. Histological analyses of the growth plate did not reveal any differences between control and mutant mice. Expression patterns of Sox9, a transcription factor required for cartilage morphogenesis, and p57, a marker of cell cycle arrest and hypertrophic chondrocyte differentiation, was unaffected. However, loss of ATRX in cartilage led to a delay in the ossification of the hips in some mice. We also observed hindlimb polydactily in one out of 61 mutants. CONCLUSIONS/SIGNIFICANCE:These findings indicate that ATRX is not directly required for development or growth of cartilage in the mouse, suggesting that the short stature in ATR-X patients is caused by defects in cartilage-extrinsic mechanisms